Jennifer Morrison was born in 1979 in Chicago, to David and Judy Morrison. She has a younger sister, Julia Kay and a younger brother, Daniel. She attended Prospect High School, and received a BA in Theater and English from Loyola University in Chicago.
She started appearing in advertisements as a child, and after graduating University, she studied with the Steppenwolf Theater Company in Chicago.
She made her film debut in 1994, and became a regular cast member of House when it premiered in 2004.