Serafinowicz made his broadcasting debut in 1993 on Radio 1 show The Knowledge, a spoof documentary about the music industry. He then went on to radio 4 and hosted a couple of shows there.
In 1999 he had made some notable appearances on the channel 4 sitcom Spaced. Peter written and stared in the short lived BBC show Look around you, and after this he moved onto the ITV sitcom Hardware.
In 2004 Peter appeared in British comedy Shaun of the Dead, he played the role of Pete, Shaun's flat-mate.
Over the years Peter has appeared in many sitcoms like, Smack the pony, I'm Alan Partridge and Black books.
In 2007 Peter created the Peter Serafinowicz show.
He has a has a child that was born in April 2007.